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Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Journal: STAR Protocols

Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

doi: 10.1016/j.xpro.2026.104471

Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

Article Snippet: Note: We use commercial flow cytometry staining buffer from eBioscience (Cat# 00-4222-26), which is PBS-based formulation designed to prevent non-specific antibody binding and maintain cell stability during flow cytometry.

Techniques: Flow Cytometry, Isolation, Staining

Synthesis, characterization, and exosomes release profile of Exos-AlgMA hydrogels. (A) Schematic diagram of the synthesis of AlgMA. (B) The 1 H NMR spectra of Alg and AlgMA. (C) Compressive stress-strain curves and (D) corresponding compressive moduli of AlgMA and Exos-AlgMA hydrogel. (n = 3; ns: no significance). (E) SEM micrograph of the porous structure of the AlgMA hydrogel. Scale bar: 50 μm. (F) TEM image of BMSC-derived exosomes. Scale bar: 50 nm. (G) Particle size distribution of exosomes measured by DLS. (H) Cumulative release profiles of exosomes from the Exos-AlgMA hydrogel over 10 days. (I, J) Bead-assisted flow cytometry analysis of exosomal markers (CD9 and CD63). Percentages indicate CD9 + or CD63 + events within the bead gate, with unstained/blank controls used to define positive thresholds.

Journal: Materials Today Bio

Article Title: The mechanistic study of injectable hydrogel loaded with BMSC-exosomes in regulating the TGF-β/MMP axis to inhibit experimental myopia model

doi: 10.1016/j.mtbio.2026.103051

Figure Lengend Snippet: Synthesis, characterization, and exosomes release profile of Exos-AlgMA hydrogels. (A) Schematic diagram of the synthesis of AlgMA. (B) The 1 H NMR spectra of Alg and AlgMA. (C) Compressive stress-strain curves and (D) corresponding compressive moduli of AlgMA and Exos-AlgMA hydrogel. (n = 3; ns: no significance). (E) SEM micrograph of the porous structure of the AlgMA hydrogel. Scale bar: 50 μm. (F) TEM image of BMSC-derived exosomes. Scale bar: 50 nm. (G) Particle size distribution of exosomes measured by DLS. (H) Cumulative release profiles of exosomes from the Exos-AlgMA hydrogel over 10 days. (I, J) Bead-assisted flow cytometry analysis of exosomal markers (CD9 and CD63). Percentages indicate CD9 + or CD63 + events within the bead gate, with unstained/blank controls used to define positive thresholds.

Article Snippet: Exosome surface markers were analyzed using a bead-assisted flow cytometry method.

Techniques: Derivative Assay, Flow Cytometry